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Centrifuge principle

When the suspension containing fine particles of standing motionless, as the role of gravity makes the gradual sinking of suspended particles. Heavier particles, sinking faster, whereas smaller particles than the liquid density will float. Particles in the gravitational field the speed of particles moving under the size, shape and density, and also with the gravitational field strength and the viscosity of the liquid. As the size of the particles of red blood cells, number of microns in diameter, can be observed in normal gravity to their settlement process.

In addition, the settlement of material in the medium Shihai accompanied by diffusion. Diffusion is unconditional and absolute. Diffusion is inversely proportional to the quality and substance, the smaller the particles spread more serious. The settlement is relative, conditional, subject to external forces in order to campaign. Settlement with the weight of the object is proportional to the larger particles settling faster. For less than a few microns, such as viruses or proteins, which in solution into a colloidal or semi-colloidal state, it is impossible to observe using only gravity to the settlement process. Because the smaller the particle the slower the sedimentation, and diffusion is more serious. So need to use a strong centrifugal force centrifuge, in order to force these particles have to overcome the spread of the settlement movement.

Centrifugation is the use of high-speed rotation of the centrifuge rotor powerful centrifugal force, to accelerate the liquid in the particle settling velocity, the sample in the sedimentation coefficient and buoyant density of different substances separate.
 

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